| General description | Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Aliquots of 4 x 50µl are provided to prevent the need for excessive freeze/thaw, and maximize functional viral particles. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at RNAi@sial.com. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set. |
| Other Notes | The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated. |
| | Number of Genes: 10, Number of Clones: 55 |
| | For a detailed listing of other available gene family sets, visit the gene family set website. |
| | Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene. |
| Legal Information | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense . |
| | MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
